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O. T. Ojo
N. G. Olaiya


Pre-treatment technique of biomass for cellulose nanofiber isolation is very significant on its properties. Several techniques have been used for pre-treatment of biomass, however much has not been done on the use of combined pre-treatment for Cellulose Nanofibre (CNF) isolations. The use of enzymes has been attached to the extraction of ethanol from biomass. In this study, a critical review of the application of combine mechanical-enzymes in isolation of CNF was discussed. A parametric study of factors that influence the output of mechanical and enzymatic hydrolysis was done with reference to previous work in this area. The review concluded by assessing the viability of enzymatic hydrolysis in combination with other techniques for successful isolation of cellulose nanofibre

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